Search for: "polymerase chain reaction" - 78 articles found

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Sponsored • Tools for the lab

Speeding up diagnostics to detect antibiotic resistance

Infectious disease diagnostics are notoriously slow. The gold standard for laboratory diagnosis of bacterial and fungal infection involves growing the pathogen from a clinical specimen – an overnight event, or even longer. The healthcare focus is on improving the use of antibiotics for better patient outcomes and reducing the environmental pressures that drive antibiotic resistance. To impact…

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News • Coronavirus disease biomarkers

New Covid-19 testing method gives results within one second

The Covid-19 pandemic made it clear technological innovations were urgently needed to detect, treat, and prevent the SARS-CoV-2 virus. A year and a half into this epidemic, waves of successive outbreaks and the dire need for new medical solutions — especially testing — continue to exist. In the Journal of Vacuum Science & Technology B, researchers from the University of Florida and…

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News • Coronavirus diagnostics

Rapid Covid-19 test delivers results within 4 minutes with 90 percent accuracy

A low-cost, rapid diagnostic test for Covid-19 developed by Penn Medicine provides Covid-19 results within four minutes with 90 percent accuracy. A paper published this week in Matter details the fast and inexpensive diagnostic test, called RAPID 1.0 (Real-time Accurate Portable Impedimetric Detection prototype 1.0). Compared to existing methods for Covid-19 detection, RAPID is inexpensive and…

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Article • dPCR and HSAFM

Low-cost technique for missed genetic mutations

A new low-cost method targeting genetic mutations often missed by existing diagnostic approaches has been developed. Researchers at Virginia Commonwealth University (VCU) in the United States noted that most rearrangement mutations implicated in cancer and neurological diseases fall between what can be detected by DNA sequence reads and optical microscopy methods. The new technique combines…

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News • Reverse genetics system

SARS-CoV-2: An easier and quicker way to analyse mutations

SARS-CoV-2 is the virus responsible for the Covid-19 pandemic. We know that mutations in the genome of SARS-CoV-2 have occurred and spread, but what effect do those mutations have? Current methods for studying mutations in the SARS-CoV-2 genome are very complicated and time-consuming because coronaviruses have large genomes, but now a team from Osaka University and Hokkaido University have…

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News • SARS-CoV-2 detection and sequencing

'Nirvana': Fast, portable test diagnoses Covid-19, tracks variants

Clinicians using a new viral screening test can not only diagnose Covid-19 in a matter of minutes with a portable, pocket-sized machine, but can also simultaneously test for other viruses—like influenza—that might be mistaken for the coronavirus. At the same time, they can sequence the virus, providing valuable information on the spread of Covid-19 mutations and variants. The new test, dubbed…

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Article • Mass spectrometry analysis

Skin swabs can detect Covid-19

Skin swab samples analysed using mass spectrometry could be used to detect Covid-19 in patients, according to research conducted at the University of Surrey in the UK. Current Covid-19 testing is via a polymerase chain reaction (PCR) test, which involves taking a swab of the back of the throat and inside the nose, but the team from Surrey - working with Frimley NHS Trust and the Universities of…

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News • Improved Coronavirus detection

Low-cost and fast Covid-19 test uses pool of gargle lavage samples

In order to monitor and contain the spread of SARS-CoV-2 it is necessary to test large numbers of people on a regular basis in decentralized settings. Researchers of the Max Planck Institute for Evolutionary Anthropology and the Hospital St. Georg in Leipzig, Germany, have developed improved protocols for the detection of SARS-CoV-2. The method can detect a positive sample in a pool with 25…

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Sponsored • Wako β-glucan test

Better detection and diagnosis of fungal infections

Beta-glucan tests are proving to be pivotal in the better detection and diagnosis of fungal infections. As a robust complimentary test for traditional testing techniques and biomarkers, it is helping clinicians deliver rapid results and offering greater reassurance in more accurately identifying such infections. β-glucan testing, which is an in vitro diagnostic test, is regularly used at…

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News • Study from Zambia shows many more deaths

Experts: Impact of Covid-19 in Africa “vastly underestimated”

The impact of Covid-19 in Africa has been vastly underestimated, warn researchers in a new study. Outside of South Africa, this is the first study to provide systematic surveillance data capturing the impact of Covid-19 in Africa. Their findings are based on polymerase chain reaction (PCR) test results for 364 deceased people of all ages at the University Teaching Hospital morgue in Lusaka,…

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News • Image-based diagnosis of Covid-19

AI detects coronavirus on CT scans

In order to detect the Corona virus SARS-CoV-2, there are further methods of diagnosis apart from the globally used PCR tests (Polymerase chain reaction): The infection can also be recognised on CT scans – for which Artificial Intelligence (AI) can be used as well. An AI system can not only filter CT scan of Covid-19 patients from a data set, but also estimate, which areas of the image are of…

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News • New microscopy approach

A smartphone-based test for Covid-19

Researchers at the University of Arizona are developing a Covid-19 testing method that uses a smartphone microscope to analyze saliva samples and deliver results in about 10 minutes. The research team, led by biomedical engineering professor Jeong-Yeol Yoon, aims to combine the speed of existing nasal swab antigen tests with the high accuracy of nasal swab PCR, or polymerase chain reaction,…

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Article • Glioma detection

Breakthrough liquid biopsy test to detect mutations in brain tumours

Researchers at Massachusetts General Hospital (MGH) in Boston have developed a novel blood test using an enhanced form of liquid biopsy capable of detecting the most common types of genetic mutations that occur in glioma brain tumors. The test is easy to use, inexpensive, produces results rapidly, and can be performed in most clinical laboratories. The researchers believe that the blood test has…

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Article • Coronavirus imaging

Covid-19: Is CT more sensitive than PCR testing?

Covid-19 causes characteristic changes in lung tissue visible in CT scans and chest radiographs, known as “ground-glass” opacities. Imaging is now considered a valid alternative, possibly even superior to RT-PCR. ‘This sparked an international debate about the role of CT in the diagnostic work-up of Covid-19,’ said radiologist Professor Cornelia Schäfer-Prokop.

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Article • The role of chest CT in diagnosis and treatment

UPDATE: Covid-19 and lung infections imaging

RSNA 2020: International experts showcased new studies on chest CT’s role in Covid-19 diagnosis and treatment. A staggering volume of work and has been produced on the pandemic this year, with an average 367 Covid-19 journal articles published per week, according to Michael Chung, Assistant professor of radiology at Icahn School of Medicine at Mount Sinai, NYC.

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News • New gargle test

Mass spectrometry to improve Covid testing

A UK biotech laboratory has used mass spectrometry in a new approach to coronavirus testing. MAP Sciences developed a gargle test, which collects samples from the back of the throat, and avoids the unpleasant sensation of the current PCR (polymerase chain reaction) swab tests. From there, the sample is tested for coronavirus using mass spectrometry (MS) with high levels of accuracy.

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Article • MEDICA session

PCR tests excel during Covid-19 pandemic

In early 1990, at Analytica, in Munich, a young US-American researcher Kary B Mullis received the award for biochemical analytics for his 1983 invention: the polymerase chain reaction (PCR) – a success topped in 1993 by the Nobel Prize for chemistry. Mullis’ work revolutionised DNA copying, a process which, before PCR, had taken weeks. Whilst initially PCR was used to create digital…

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News • False positive results

Accuracy of rapid covid test may be lower than previously suggested

The accuracy of a rapid finger-prick antibody test for SARS-CoV-2, the virus responsible for covid-19 infection, may be considerably lower than previously suggested, finds a study published by The BMJ. The results suggest that if 10% of people given the test had previously been infected, around 1 in 5 positive test results would be incorrect (false positive results). These conclusions contrast…

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News • Using virus particles in exhaled breath

New SARS-CoV-2 test to deliver results in under 5 minutes

Research and innovation hub Imec announced that it has started developing a groundbreaking SARS-CoV-2 test. Unlike current approaches (using blood, saliva, or a nasopharyngeal swab), the new test will identify SARS-CoV-2 virus particles in a person’s exhaled breath. The solution promises the accurate identification of a contagious case in less than five minutes.

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Article • Corona management in Taiwan

Standing united against COVID-19

Despite its proximity to China, Taiwan contained COVID-19 successfully, without a lockdown or movement restriction measures introduced elsewhere. With few new cases reported, life almost returned to normal. Behind the scenes, however, efforts have continued to maintain that positive situation.

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News • COVID-19 detection

New analyzer detects virus antibodies in 20 minutes

Researchers at Hokkaido University have succeeded in detecting anti-avian influenza virus antibody in blood serum within 20 minutes, using a portable analyzer they have developed to conduct rapid on-site bio tests. If a suitable reagent is developed, this technology could be used to detect antibodies against SARS-CoV-2, the causative virus of COVID-19.

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News • What radiologists need to know

How lung disorders like COVID-19 affect children

Although the clinical symptoms of new pediatric lung disorders such as severe acute respiratory syndrome (SARS), swine-origin influenza A (H1N1), Middle East respiratory syndrome (MERS), e-cigarette or vaping product use-associated lung injury (EVALI), and coronavirus disease (COVID-19) pneumonia may be nonspecific, some characteristic imaging findings "have emerged or are currently…

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Article • Coronavirus countermeasures

Learning from China – the role of radiology in combatting COVID-19

As the first country to be hit by COVID-19, China learned a number of early lessons into how to combat the highly-infectious disease. With radiology teams playing an important role and utilising CT chest scans as a diagnostic tool against coronavirus, Chinese practitioners have found themselves well-placed to offer a valuable insight on how to combat and contain COVID-19. In a special webcast –…

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News • COVID incidence at airports and in hospitals

Biosensor to detect coronavirus in crowded places

A team of researchers from Empa, ETH Zurich and Zurich University Hospital has succeeded in developing a novel sensor for detecting the new coronavirus. In future it could be used to measure the concentration of the virus in the environment - for example in places where there are many people or in hospital ventilation systems. Jing Wang and his team at Empa and ETH Zurich usually work on…

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News • Volunteering against the coronavirus

Scientists on Standby portal to support COVID-19 testing recruitment

Scientists on Standby has been rapidly conceptualised and launched in response to the COVID-19 crisis and in anticipation of the increasing pressure on UK diagnostic testing laboratories and facilities in the coming weeks and months. The portal enables scientists with relevant skills and experience to volunteer their time, expertise and support for COVID-19 screening and testing.

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News • COVID-19 diagnostics

Corona 'pool testing' increases worldwide capacities many times over

Researchers at the German Red Cross Blood Donor Service in Frankfurt headed by Professor Erhard Seifried, and the Institute for Medical Virology at the University Hospital Frankfurt at Goethe University headed by Professor Sandra Ciesek succeeded in developing a procedure that makes it possible to immediately and dramatically increase worldwide testing capacities for detecting SARS-CoV-2.

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Interview • Chest X-ray, CT and more

Imaging the coronavirus disease COVID-19

Chest X-ray is the first imaging method to diagnose COVID-19 coronavirus infection in Spain, but in the light of new evidence this may change soon, according to Milagros Martí de Gracia, Vice President of the Spanish Society of Radiology (SERAM) and head of the emergency radiology unit at La Paz Hospital in Madrid, one of the hot spots for viral re-production of COVID-19.

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News • COVID-19 in radiology

CT outperforms lab diagnosis for coronavirus infection

In a study of more than 1,000 patients published in Radiology, chest CT outperformed lab testing in the diagnosis of 2019 novel coronavirus disease (COVID-19). Researchers at Tongji Hospital in Wuhan, China, concluded that CT should be used as the primary screening tool for COVID-19. In the absence of specific therapeutic drugs or vaccines for COVID-19, it is essential to detect the disease at an…

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News • Deadly virus

New test to identify and control Ebola variants

The situation is extraordinary: there have only ever been four declarations of public health emergencies of international concern in the past and now there are two at the same time. Whilst the risks associated with the novel coronavirus are still unclear, people in the Democratic Republic of the Congo are still battling with an outbreak of the deadly Ebola virus which has been ongoing since 2018…

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News • Lab equipment

New high-precision digital reagent dispensers launched at SLAS 2020

Scientists working across drug metabolism and pharmacokinetics, bioanalytical, pre-clinical and clinical trial applications can now access a range of digital reagent dispensers designed to enable assay miniaturization, boost productivity and improve accuracy of results. Offering enhanced dispensing precision, the new systems use ten times less reagent compared to manual pipetting workflows,…

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News • Respiratory failure and sepsis

Cheap, quick test identifies major risks for pneumonia patients

Spanish researchers in Valencia have identified specific fragments of genetic material that play a role in the development of respiratory failure and sepsis in pneumonia patients. Presenting the research at the European Respiratory Society International Congress, Dr Francisco Sanz said the findings could enable doctors to test quickly for these biological markers when a patient is admitted to…

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Article • Methods, quality assurance, commercial providers issues

Molecular testing takes a huge leap

In terms of success in revolutionary cancer treatment, molecular genetic examination procedures have developed immensely over recent years. They now range from conventional polymerase chain reactions (PCR) or fluorescence-in-situ hybridisation (FISH) to Next Generation Sequencing (NGS) with analysis of the entire exome or genome (Whole-Exome, WES or Whole-Genome, WGS) and of the transcriptome…

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News • Diagnostics

Heat it and read it

You’re sweating and feverish and have no idea why. Fortunately, Sandia National Laboratories scientists have built a device that can pinpoint what’s wrong in less than an hour. Unlike most medical diagnostic devices which can perform only one type of test — either protein or nucleic acid tests — Sandia’s SpinDx can now perform both. This allows it to identify nearly any cause of…

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News • Bordetella

Launch of Hologic's whooping cough detection assay in Europe

Hologic, Inc. announced that its Panther Fusion Bordetella assay has received CE mark in Europe. This assay, the latest in a growing menu of Panther Fusion and Aptima assays, brings full automation, efficiency and excellent assay performance to Bordetella (whooping cough) detection. The Panther Fusion system retains all the key benefits of the Panther platform, including full sample-to-result…

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Article • Infection control

Global warming: Science can only react to emerging diseases

The ‘Transmission, Prevention, and Reporting of Emerging Infectious Diseases’ program for the International Conference IMED 2016 in Vienna, this November, reflected events in the field of emerging diseases that have occurred over the last two years. Therefore, key congress topics included the Zika virus, the effects of global warming and the unusually high number of hospital-acquired…

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Article • Infections

Science can only react

The ‘Transmission, Prevention, and Reporting of Emerging Infectious Diseases’ programme for the International Conference IMED 2016 in Vienna, this November, reflected events in the field of emerging diseases that have occurred over the last two years.

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Article • Frontline medical advances

Virology is now a key discipline

Virology is fast emerging as a key discipline within modern healthcare against a backdrop of a shifting global demographic and the impact of climate change.

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News • Lung cancer

“Liquid biopsy” blood test detects genetic mutations

A simple blood test can rapidly and accurately detect mutations in two key genes in non-small cell lung tumors, researchers at Dana-Farber Cancer Institute and other institutions report in a new study – demonstrating the test’s potential as a clinical tool for identifying patients who can benefit from drugs targeting those mutations.

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Novel DNA repair mechanism brings new horizons

A group of researchers, lead by Vasily M. Studitsky, professor at the Lomonosov Moscow State University, discovered a new mechanism of DNA repair, which opens up new perspectives for the treatment and prevention of neurodegenerative diseases. The article describing their discovery is published in AAAS' first open access online-only journal Science Advances.

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News • Microscope technique

Speeding identification of deadly bacteria

A new way of rapidly identifying bacteria, which requires a slight modification to a simple microscope, may change the way doctors approach treatment for patients who develop potentially deadly infections and may also help the food industry screen against contamination with harmful pathogens, according to researchers at the Korea Advanced Institute of Science and Technology (KAIST) in Daejeon,…

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Article • IVD Market

Santa’s bag full of cash

We are seeing great priority shifts in China’s funding for R&D and manufacturing expansion. Even the agency responsible for selecting the recipients has changed, Jie Ren reports from the Beijing-based market analysis consultancy Whitney Research Inc. Report: John Brosky

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News • Infectious Diseases

Abbott’s Testing Platform Iridica Now Available in Europe

Abbott announced its innovative diagnostic platform, known as Iridica, is now available in Europe and other CE–Mark recognized countries. The new platform can identify more than 1,000 infection–causing pathogens in less than six hours.

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Article • Medical Training

Diagnosing gastrointestinal infections

The human gut literally teems with microorganisms from at least 1,000 different species that are increasingly considered to be a valuable resource for the prediction, aetiology and prognosis of disease. Due to continual contact with the environment, primarily via food, the gut is susceptible to infection when a virus, parasite or bacterium enters and disrupts normal gut microbiota (or flora).

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A new strain of MRSA discovered

While researching bovine mastitis (an S. aureus infection that occurs in the cows’ udders), researchers led by Dr Mark Holmes at the University of Cambridge, UK, identified a new strain of methicillin-resistant Staphylococcus aureus (MRSA) which occurs both in human and dairy cow populations.

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The 3rd Annual General Meeting of the Austrian Society for Laboratory Medicine and Clinical Chemistry

Different tests bring different results, and results of the same types of test vary from laboratory to laboratory. "In the case of test procedures for autoimmune diseases there are incredible discrepancies," confirmed Professor Manfred Herold, head of the Laboratory for Rheumatism at the University Clinic for Internal Medicine One, Innsbruck. The reason: "There are no standards for…

Cancer screening made simple

Current cervical cancer screening is time consuming and expensive, but now new breakthrough technology developed by European researchers should allow large-range screening by non-medical personnel with almost immediate results and at a much lower cost.

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Article • MRSA

Effective screening can kill several birds with one stone

The MRSA problem has been ignored almost stoically in many European countries for the past 20 years. Thus the number of resistant Staphylococci cases exploded from 1% in 1990 around 25% in 2010. However, the recognition that an infection can result in additional hospital costs of up to €10,000 has led to a change in thinking. The Netherlands, for example, declared war on MRSA with ‘search and…

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C. difficile - Molecular scientists design a very quick cheap test

A small team of researchers at Barts and London School of Medicine and Dentistry, in London, has developed a rapid, cheap and effective kit to test for the presence of Clostridium difficile. And, they report that the new kit costs as little as 50 pence to £1 per test and can deliver results in under an hour, compared to commercial kits that cost up to £25 and in some cases take significantly…

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The value and development of PCR tests

Whereas, in the past, the diagnoses of infectious diseases focused on antibody detection, more recently laboratories aim to identify the agent itself, using the invaluable PCR (polymerase chain reaction) test. We asked Prof Beatus Ofenloch-Hähnle, head of Reagent and System Development Immunology, at Roche Professional Diagnostics, and Benjamin Lilienfeld, Global Product Manager for Infectious…

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Nosocomial infections

According to ECD statistics for Europe, three million cases of nosocomial infections occur annually, and 50,000 are fatal. Evelina Tacconelli MD PhD (below) is Assistant Professor of Infectious Diseases at the Università Cattolica Sacro Cuore in Rome, Italy. Her scientific focus is on epidemiology, clinical and therapeutic aspects of nosocomial infections and infection control policies aimed to…

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