News • Multiplex immunoassay

Dengue Fever: hope for low-cost diagnostics

Accurate home testing could be used for a wider range of illnesses, as new research shows the capability of smartphone-powered tests for Dengue Fever.

Photo
Researchers created a rapid multiplex immunoassay for the detection of Dengue fever.
Source: University of Reading

Biomedical technology researchers from the University of Reading used a new diagnostic kit called Cygnus to detect Dengue Fever with significantly improved rates over lateral flow testing kits.

Working with academics and clinicians in Thailand, the team trialled the tests alongside already established alternatives in and found the new tests showed 82% clinical sensitivity, beating lateral flow testing (74% sensitivity) and matching hospital-based lab diagnostics (83% sensitivity). At the same time, these devices make 10 measurements allowing us to identify which of the 4 different dengue virus types caused the infection.

Dr Sarah Needs, Postdoctoral Research Associate in Microfluidic Antimicrobial Resistance Testing from the University of Reading is lead author of the paper. “The paper shows exciting potential for the use of the microfluidic ‘lab on a strip’ tests that can used in conjunction with a smartphone and are more powerful than LFT testing in this case. As well as being cheap to produce, the lab on a strip technology allows users to test many different targets at once in one single sample, so it could be useful to detect multiple diseases not just one," she said.

Lab on a strip

The new diagnostic test developed for the research uses ‘lab on a strip’ technology, which performs 10 or more tests a very small amount of liquid sample (such as blood, urine or saliva).

The tests developed for the research were specifically developed to detect Dengue Fever, which affects an estimated 400m cases each year. While most cases are mild, dengue infections can lead to significant complications and can be fatal. Dengue can be most severe in children and is a serious health challenge facing half the global population.

Dr Alexander Edwards, Associate Professor in Biomedical Technology at the University of Reading co-created the lab on a strip technology.
“While some people might only recently learned of the trade-offs between home vs lab testing following Covid-19, in many parts of the world rapid lateral flow tests are used for a range of illnesses including dengue," Edwards said. “With the Cygnus concept, we are tackling the biggest hurdle for home testing. How do you make something portable that can be cheaply mass produced while still matching laboratory test performance? By designing the microfluidic lab on a strip using mass-production melt-extrusion it is possible to scale up production and produce hundreds of thousands of tests. By recording results with smartphones, which are becoming ubiquitous, we have designed something that could be revolutionary for healthcare.”

Research was published in PLOS Neglected Tropical Diseases.

Source: University of Reading

22.04.2022

Read all latest stories

Related articles

Photo

Chemistry & immunochemistry

Fujifilm Wako – Autokit CH50 Assay

Autokit CH50 Assay is an in vitro diagnostic (IVD) test for the quantitative determination of total complement activity in human serum. The complement system is a part of the immune system that…

Photo

News • COVID-19 diagnostics

SARS-CoV-2 IgG antibody test receives FDA Emergency Use Authorization

Beckman Coulter announced that its Access SARS-CoV-2 IgG assay has received Emergency Use Authorization (EUA) from the U.S. Food & Drug Administration (FDA). Beckman Coulter has already shipped…

Photo

News • Infectious disease

Sensor technology could detect Dengue fever earlier

Researchers from the University of Bath are developing a new tool for detecting the presence of Dengue fever early on, helping prevent people from suffering potential life-threatening complications.

Related products

Beckman Coulter – SARS-CoV-2 Assays

Immunoassays

Beckman Coulter – SARS-CoV-2 Assays

Beckman Coulter Diagnostics
Mindray – CL-6000i Chemiluminescence Immunoassay System

Immunoassays

Mindray – CL-6000i Chemiluminescence Immunoassay System

Shenzhen Mindray Bio-Medical Electronics Co., Ltd
Snibe - Maglumi X3

Immunoassays

Snibe - Maglumi X3

Snibe Diagnostic
Beckman Coulter – Access 2 Immunoassay System

Immunoassays

Beckman Coulter – Access 2 Immunoassay System

Beckman Coulter Diagnostics
Subscribe to Newsletter