When heated, the proteins in the originally transparent chicken egg white form a tightly meshed, opaque network

Credit: DESY, Gesine Born

News • Innovative protein analysis

Cooking an egg in an X-ray beam

A team of scientists has been using the X-ray source PETRA III at the German Electron Synchrotron (DESY) to analyse the structural changes that take place in an egg when you cook it. The work reveals how the proteins in the white of a chicken egg unfold and cross-link with each other to form a solid structure when heated.

Their innovative method can be of interest to the food industry as well as to the broad field of research surrounding protein analysis. The cooperation of two groups, headed by Frank Schreiber from the University of Tübingen and Christian Gutt from the University of Siegen, with scientists at DESY and European XFEL reports the research in two articles in the journal Physical Review Letters. (1) (2)

Eggs are among the most versatile food ingredients. They can take the form of a gel or a foam, they can be comparatively solid and also serve as the basis for emulsions. At about 80 degrees Celsius, egg white becomes solid and opaque. This is because the proteins in the egg white form a network structure. Studying the exact molecular structure of egg white calls for energetic radiation, such as X-rays, which is able to penetrate the opaque egg white and has a wavelength that is no longer than the structures being examined. “To understand the structural evolution in detail, you have to study the phenomenon on the micrometre scale,” explains Nafisa Begam, the lead author of the first study, who is an Alexander von Humboldt fellow in Schreiber’s group. The scientists used so-called X-ray photon correlation spectroscopy (XPCS) with a specific geometry allowing them to determine the structure and the dynamics of the proteins in the egg white.

Beamline P10 at DESYs X-ray source PETRA III where the experiments took place
Beamline P10 at DESY's X-ray source PETRA III where the experiments took place

Credit: University of Tübingen, Anastasia Ragulskaya

For their experiments on the P10 beamline at PETRA III the scientists used a chicken egg from a supermarket and filled the egg white into a quartz tube with a diameter of 1.5 millimetres. “Inside, the egg white was heated in a controlled manner while we analysed it with the help of the X-rays,” explains DESY co-author Fabian Westermeier. “The X-ray beam was expanded to 0.1 by 0.1 millimetres, to keep the radiation dose below the damage threshold of the protein structures.” The measurements reveal the protein dynamics in the egg white over a period of about a quarter of an hour. During the first three minutes, the protein network grew exponentially, reaching a plateau after about five minutes, at which virtually no more protein links were formed. At this time, the average mesh size of the protein network was about 0.4 micrometres (thousandths of a millimetre).

Successfully applying X-ray photon correlation spectroscopy opens up a new way to study the dynamics of biomolecules, which is essential if we are to understand them properly

Frank Schreiber

In the second study, the team used the XPCS technique to investigate the self-organisation of protein solutions into domains with, respectively, high and low protein concentration, as an example of structure formation in cell biology. In the process, they were able to follow the temperature-dependent dynamics over time. “At high protein densities, mobility decreases, which slows down the phase separation. This is important for the special dynamics of the system,” reports lead author Anita Girelli from Schreiber’s group.

The studies, which were funded by the German Federal Ministry of Education and Research (BMBF), not only reveal new details about the structural changes occurring in egg whites, but also prove the experimental concept, which can be used for other samples too, as demonstrated by the second study. “Successfully applying X-ray photon correlation spectroscopy opens up a new way to study the dynamics of biomolecules, which is essential if we are to understand them properly,” Schreiber comments.


Source: Deutsches Elektronen-Synchrotron

04.04.2021

Read all latest stories

Related articles

Photo

News • Coronavirus diagnostic distribution

Drones as a delivery system for Covid-19 tests?

Researchers have developed a new Covid-19 test distribution method that uses drones to quickly deliver at-home diagnostic tests to individuals who suspect they have Covid-19.

Photo

Sponsored •  

Preanalytical challenges and their clinical impact in point-of-care testing

The European Society for Emergency Medicine (EUSEM) held their annual congress on October 27th to 31st, 2021 in the city of Lisbon, to promote education, training and research in European acute care.

Photo

Sponsored • Handheld diagnostics

Veros™ COVID-19 test now available in Europe

Handheld, instrument-free test delivers lab-quality molecular results – 97.9% accuracy – in about 15 minutes, right at the point of care.

Related products

Beckman Coulter – Access 2 Immunoassay System

Immunoassays

Beckman Coulter – Access 2 Immunoassay System

Beckman Coulter Diagnostics
Beckman Coulter – DxA 5000

Automation

Beckman Coulter – DxA 5000

Beckman Coulter Diagnostics
Beckman Coulter – SARS-CoV-2 Assays

Immunoassays

Beckman Coulter – SARS-CoV-2 Assays

Beckman Coulter Diagnostics
Dirui – CM-180 Chemiluminescence Immunoassay Analyzer

Immunoassays

Dirui – CM-180 Chemiluminescence Immunoassay Analyzer

Dirui Industrial Co.,Ltd.
Dirui – FUS-1000 Urinalysis Hybrid

Urine Screening

Dirui – FUS-1000 Urinalysis Hybrid

Dirui Industrial Co.,Ltd.
Subscribe to Newsletter