Image source: Daniel Roberts from Pixabay

Enzyme Linked Immunosorbent Assay

Startup develops breakthrough IgG antibody test for Covid-19

ZTA Biotech, a Budapest-based biotech startup, has developed a Covid-19 antibody (IgG) test using the ELISA protocol. This new detection method represents a great step forward in determining if patients have had a coronavirus infection and if they might still have immunity to the disease.

Early results have proven 100% in specificity after testing 280 samples, and 100% sensitivity by testing 260 samples of recovered and symptomatic patients.

With a potential market of 5.8 billion people, who will likely need multiple antibody tests in the coming years, the formula has a massive potential across the globe. ZTA Biotech is currently offering the intellectual property rights to licensees and/or to a sole-rights purchaser, in order to help bring this breakthrough ELISA formula to market. Given the relatively small number of ELISA-based protocols when compared to the global demand, the sale offers a unique opportunity in combatting the Covid-19 pandemic, since it gives a greater detail into the prevalence of the disease. Also, brokering services of specialized companies are encouraged to facilitate an open and transparent dialogue between ZTA Biotech and potential clients.

The presented ELISA (Enzyme Linked Immunosorbent Assay) protocol is designed for the intended use in the post-vaccination period that is soon to commence (first/second trimester of 2021). Its microplates are coated with critical antigens which are the prime targets of the current vaccine developments (of any kind). The kit can also be used to monitor past, natural exposure to SARS-CoV-2 and to evaluate the possibility of re- infection.

Recommended article

Photo

Preclinical data

COVID-19 vaccine candidate designed via computer

An innovative nanoparticle vaccine candidate for the pandemic coronavirus produces virus-neutralizing antibodies in mice at levels ten-times greater than is seen in people who have recovered from COVID-19 infections. Designed by scientists at the University of Washington School of Medicine in Seattle, the vaccine candidate has been transferred to two companies for clinical development.

The test is based on selective detection of human IgG antibodies that bind to SARS- CoV-2 antigens coated on the wells of the microplate and can be used for qualitative and semi-quantitative determination of human IgG. After the completion of the first critical reaction step, all unbound serum components are fully washed away and a series of reactions occur that will lead to a photometrically measurable step where the intensity of the obtained color is proportional to the quantity of SARS-CoV-2 IgG antibodies in the original sample. The whole process is fast, takes less than 2 hours to complete and can be fully automated. Also, it can be easily converted to a quantitative test, if needed.

The test was independently validated, with a sensitivity of 100% and a specificity of 100%, and compared to an approved test of a major test producer, the latter being outperformed. All testing for validation was carried out on samples collected between September 2019 – June 2020. The antibody test showed extremely low background noise, technical data available upon request. All sensitivity evaluation data was based on PCR tested, positive SARS-CoV-2 patients, all symptomatic after getting infected. No cross-reactivity was observed to SARS- CoV-2 IgM. The kit is extremely stable, has a long shelf life and opened kits are usable up to 3 months at storing temperatures of 5°C.

Initial equipment and personnel costs are low and are proportional to the desired monthly production. Also, the test can be adapted to potential virus mutations, based on sequencing data.


Source: ZTA Biotech

06.01.2021

Read all latest stories

Related articles

Photo

Coronavirus antigen detection

An innovative test for the early diagnosis of Covid-19

Compared to previous SARS and MERS, SARS-CoV-2 became a pandemic due to the high infectivity and different mode of contagion. SARS-CoV-2 spreads more because it is contagious also before symptoms,…

Photo

Immune response study

Is a "natural" Covid-19 infection better than vaccination? It's complicated

Hope for a future without fear of Covid-19 comes down to circulating antibodies and memory B cells. Unlike circulating antibodies, which peak soon after vaccination or infection only to fade a few…

Photo

Immunological memory

How our lungs 'remember' a Covid-19 infection

After infection with SARS-CoV-2, where does the immune system store the memory to provide long-term protection against reinfection? Though numerous studies have examined blood to track immune…

Related products

Beckman Coulter – SARS-CoV-2 Assays

Immunoassays

Beckman Coulter – SARS-CoV-2 Assays

Beckman Coulter Diagnostics
Lifotronic – SARS-CoV-2 Nucleic Acid Detection Kit

Detection

Lifotronic – SARS-CoV-2 Nucleic Acid Detection Kit

Lifotronic Technology Co., Ltd
Mindray – CL-1000i/1200i Chemiluminescence Immunoassay System

Immunoassays

Mindray – CL-1000i/1200i Chemiluminescence Immunoassay System

Shenzhen Mindray Bio-Medical Electronics Co., Ltd
Mindray – CL-2000i Chemiluminescence Immunoassay System

Immunoassays

Mindray – CL-2000i Chemiluminescence Immunoassay System

Shenzhen Mindray Bio-Medical Electronics Co., Ltd
Mindray – CL-6000i Chemiluminescence Immunoassay System

Immunoassays

Mindray – CL-6000i Chemiluminescence Immunoassay System

Shenzhen Mindray Bio-Medical Electronics Co., Ltd