Genetic Disorder

How to predict development of autism or psychosis

Doctors and researchers have long known that children who are missing about 60 genes on a certain chromosome are at a significantly elevated risk for developing either a disorder on the autism spectrum or psychosis — that is, any mental disorder characterized by delusions and hallucinations, including schizophrenia. But there has been no way to predict which child with the abnormality might be at risk for which disorder.

Carrie Bearden, a professor of psychiatry and psychology at researcher at UCLA,...
Carrie Bearden, a professor of psychiatry and psychology at researcher at UCLA, led a team that shed the first light on the genetic differences between DiGeorge syndrome patients with autism and those with psychosis.
Source: Mark Wheeler/UCLA

New findings by researchers at UCLA and the University of Pittsburgh are the first to suggest a potential way to make that determination. In a study published in PLOS One, the researchers report having isolated specific genetic differences between people with the chromosomal deletion — known as 22q11.2 deletion syndrome or DiGeorge syndrome — who have autism and those who have psychosis.

“Ultimately, this kind of information could be used as a diagnostic tool that could allow pediatricians or other clinicians to determine who will develop which disorder, so that the appropriate intervention can be applied — and applied early enough to have the most impact,” said Carrie Bearden, the study’s senior author and a professor of psychiatry and psychology at UCLA. “We know that early intervention is very important for people at risk for autism or psychosis.”

DiGeorge syndrome affects roughly 1 in 2500 children born worldwide, and is the second most common genetic abnormality, after Down syndrome. It can be detected with an amniocentesis — a prenatal medical procedure used to check for genetic and chromosomal disorders. Children with DiGeorge syndrome often have elongated faces, almond-shaped eyes and unusual outer ears. They frequently have palate abnormalities, including cleft palate, and they are at elevated risk for cardiovascular defects.

DiGeorge syndrome is the highest known genetic risk factor for psychosis, and one of many known genetic risk factors for autism. Between 30 and 40 percent of individuals with the syndrome are diagnosed with a disorder on the autism spectrum, and between 25 and 30 percent are diagnosed with a psychotic disorder. (A small number are diagnosed with both autism and psychosis.) “The hope is that eventually we could identify individuals at risk for either disorder with a blood sample,” said lead author Maria Jalbrzikowski, who was a postdoctoral fellow in Bearden’s lab while they were conducting the study.

Bearden and Jalbrzikowski, who is now a postdoctoral fellow at the University of Pittsburgh, took blood samples from 46 UCLA patients with the deletion. They also took blood samples from 66 control subjects. They analyzed the samples using a new technique developed by UCLA geneticist Steve Horvath. Called “weighted gene co-expression network analysis,” it allows researchers to look for patterns of genes that are connected to one another. The analysis determined whether specific gene expression patterns — that is, whether specific groups of genes are activated for a given trait — were associated with psychosis or autism.

On average, people with DiGeorge syndrome and psychosis had 237 genes that showed a different pattern from the genes of people with the syndrome but without psychosis. Most of these genes were associated with the regulation of gene expression — that is, the way that genes end up being read as an individual develops. “Having one chunk of DNA missing appears to cause downstream effects, with other functions becoming disrupted,” said Bearden.

In a separate step, the researchers compared the genes associated with psychosis in the UCLA group of DiGeorge syndrome patients with psychosis to those of a sample of 180 Dutch patients who had been diagnosed with schizophrenia but did not have the syndrome. They found an overlap of seven genes. “This finding is really important because it provides proof that altered gene expression patterns in those with DiGeorge syndrome and psychosis are shared with people who are diagnosed with schizophrenia but do not have the deletion,” Bearden said. “The same pathways are affected.” The seven overlapping genes play a role in fetal brain development, suggesting that psychosis may originate during the early stages of brain development, Bearden said.

Meanwhile, the DiGeorge syndrome patients with autism differed from their counterparts without autism in the expression of 86 genes, which are likely involved in the development of the immune system.

Four years in the making and funded by the National Institute of Mental Health, the study is believed to be the first attempt at understanding the genetic differences between DiGeorge syndrome patients with autism and those with psychosis.

In future research, Bearden and Jalbrzikowski hope to replicate their findings in larger groups of patients and in other cell types (like brain tissue) in patients with DiGeorge syndrome. The approach they used — weighted gene co-expression network analysis — also holds promise for research on individuals missing genes on Chromosomes 15 and 16, the researchers said. Those individuals also are at a higher-than-normal risk of psychotic disorders, intellectual disability and epilepsy.


Source: UCLA

28.07.2015

Read all latest stories

Related articles

Photo

Benefits of country life

Kids with pets, rural upbringing become stress-resilient adults

Children raised in a rural environment, surrounded by animals and bacteria-laden dust, grow up to have more stress-resilient immune systems and might be at lower risk of mental illness than pet-free…

Photo

Early alcohol tasting

Just one sip won't hurt your child? Guess again

Parents who allow their young children to occasionally sip and taste alcohol may be contributing to an increased risk for alcohol use and related problems when those kids reach late adolescence,…

Photo

Antisocial behaviour

How the brain of children with conduct disorder is different

Behavioural problems in young people with severe antisocial behaviour – known as conduct disorder – could be caused by differences in the brain’s wiring that link the brain’s emotional…

Related products

Eppendorf – Mastercycler nexus X2

Research Use Only (RUO)

Eppendorf – Mastercycler nexus X2

Eppendorf AG
Sarstedt – Low DNA Binding Micro Tubes

Research Use Only (RUO)

Sarstedt – Low DNA Binding Micro Tubes

SARSTEDT AG & CO. KG
Shimadzu – CLAM-2030

Research Use Only (RUO)

Shimadzu – CLAM-2030

Shimadzu Europa GmbH