Testing for antibodies against SARS-CoV-2
Testing for antibodies against SARS-CoV-2

© Charité | Arne Sattler

News • New insights into antibody response

How the immune system reacts to coronavirus variants

In the journal Science, researchers from Charité – Universitätsmedizin Berlin and Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) present new findings on the immune response against the coronavirus SARS-CoV-2.

Their study, published in the journal Science, is based on investigations of antibodies elicited by infection with the Beta variant of the virus. The researchers conclude that the Beta variant can confer broad immunity to multiple viral strains, which could be beneficial for protection against the currently prevalent Delta and Omicron variants, as well as against future viral variants. In their view, this aspect should be taken into account in the development of vaccination strategies.

Recommended article

“The Beta variant of the coronavirus shows marked differences from the wild type, the original strain of the virus. Until the emergence of the now dominating Omicron variant, it was the viral form that had evolved furthest from the wild type, against which the existing vaccines have been developed,” says Dr. Momsen Reincke, a researcher at the Department of Neurology with Experimental Neurology on Campus Charité Mitte and DZNE as well as one of the first authors of the current publication. “We were interested in learning more about the exact antibody response to this variant – to see what conclusions can be drawn from this about the immune response to other variants. Since the coronavirus is likely to continue mutating, we were interested in whether the antibodies we found acted only against the Beta variant or had broader potential.” 

Antibodies are proteins that the body uses to defend itself against pathogens. The human immune system can produce an almost inexhaustible variety of them by making use of various mechanisms: in particular, by repeatedly recombining the blueprints for the components of an antibody that are stored in the genome. “The immune response to coronavirus also produces a spectrum of antibodies that bind to different areas of the pathogen,” Dr. Reincke says. From an immune defense perspective, binding to the so-called spike protein is particularly effective. “In a nutshell, this is the hook that the virus uses to attach itself to body cells so that it can then slip in. Some antibodies bind to this protein and thereby render the hook useless. These are the neutralizing antibodies. In our study, these were exactly the antibodies we looked at.”

Simultaneous or even sequential vaccination against different variants would likely provide enhanced protection against potential further forms of the coronavirus

Jakob Kreye

The findings of the Berlin scientists are based on an analysis of antibodies that they isolated from the blood of 40 individuals. All patients had been infected with the Beta variant of SARS-CoV-2. Of the approximately 300 antibodies initially surveyed, 81 bound particularly strongly to the spike protein of the coronavirus. Dr. Reincke and his colleagues unraveled the genetic blueprints of the antibodies. This enabled them to understand which genes play a role in the assembly of these antibodies and to artificially produce these immune proteins for further studies. In doing so, they benefited from a project funded by the Helmholtz Association: the 'BaoBab Innovation Lab'. Within the framework of BaoBab, they develop and refine technologies for the characterization and production of antibodies. “We tested if antibodies against the Beta variant are also effective against other virus variants. This is called cross-reactivity. Our analyses show that some of these antibodies do little against the wild type. Others, however, are very effective against the original virus strain and also against some of the Variants of Concern. Some of the antibodies against Beta are even effective against the currently circulating variants Delta and Omicron,” says Dr. Jakob Kreye, last author of the study and a scientist at Charité’s Department of Pediatric Neurology and the Department of Neurology with Experimental Neurology as well as DZNE. 

The key to cross-reactivity is the site of the spike protein a particular antibody binds to and whether this site has changed between viral variants. “The antibodies with broad efficacy target areas of the spike protein that have remained largely the same in the previous viral variants,” Dr. Kreye says. However, there are exceptions to this in the case of Omicron. “Yet we have found antibodies that work well against both Beta and Omicron and only weakly against other variants. These particular antibodies bind to sites of the spike protein that are quite similar in Beta and Omicron but not in other variants.” 

Cross-reactivity could prove to be an important aspect of future vaccinations: “Individual antibodies against the wild type also have broad efficacy. This has been described in the literature and is also shown by studies in our laboratory. Taking together these data and our current findings, we conclude that antibodies generated against different viral variants can complement each other and thus jointly improve the potency of the immune response against emerging variants. Largest possible diversity in the antibody response seems to make sense,” says Prof. Dr. Harald Prüss, senior physician at the Department of Neurology with Experimental Neurology on Campus Charité Mitte and research group leader at DZNE. Dr. Kreye adds: “Simultaneous or even sequential vaccination against different variants would likely provide enhanced protection against potential further forms of the coronavirus. This approach could be relevant for the further development of vaccination strategies, because the pathogen can be expected to keep changing in the future.” 


Source: Charité – Universitätsmedizin Berlin / Deutsches Zentrum für Neurodegenerative Erkrankungen

26.01.2022

Read all latest stories

Related articles

Photo

News • Protein malformation

Covid-19: immune reaction may cause amyloid generation

In patients with serious and long-term Covid-19, disturbed blood coagulation is often observed. Now, Swedish researchers found a connection between harmful amyloid production and Covid-19 symptoms.

Photo

News • Coronavirus research

Why the Omicron variant causes less severe disease

A new study shows that the SARS-CoV-2 Omicron variant is less effective than Delta at blocking a cellular defence mechanism against viruses, the so-called 'interferon response'.

Photo

News • Sensitivity and response detection

How long do tests detect SARS-CoV-2 antibodies? New insights

How long can Covid-19 antigen tests reliably tell the antibody status? Researchers at the Paul-Ehrlich-Institut identified several factors that affect the detection duration.

Related products

Beckman Coulter – Access Procalcitonin (PCT)

Immunoassays

Beckman Coulter – Access Procalcitonin (PCT)

Beckman Coulter Diagnostics
Beckman Coulter – SARS-CoV-2 Assays

Immunoassays

Beckman Coulter – SARS-CoV-2 Assays

Beckman Coulter Diagnostics
Fujifilm Wako – μTASWako i30

Immunoassays

Fujifilm Wako – μTASWako i30

FUJIFILM Wako Chemicals Europe GmbH
Lifotronic  SARS-CoV-2 Antibody Detection Kit

Immunoassays

Lifotronic SARS-CoV-2 Antibody Detection Kit

Lifotronic Technology Co., Ltd
Subscribe to Newsletter